Pierce™ Recombinant Protein A/G
Supplier: LIFE TECHNOLOGIES MS
Purified (unconjugated) Thermo Scientific Pierce Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.
- Contains four Fc-binding domains from Protein A and two from Protein G
- Provides binding for all antibody species and subclasses recognised by either Protein A or Protein G
The extended Fc-binding properties of Protein A/G make it a tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin. Protein A/G is an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonal are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G.
Properties of Recombinant Protein A/G:
Source: E. coli
Molecular weight: 50,460 (Apparent MW by SDS-PAGE: 40,000 to 45,000)
Form: salt-free powder
A280 of 0.1% solution: 0.505
Isoelectric point (pI): 4.65
Precaución: For Research Use Only. Not for use in diagnostic procedures.
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