Imject™ Maleimide-Activated Blue Carrier™ Protein, Thermo Scientific
Supplier: LIFE TECHNOLOGIES MS
Thermo Scientific Imject Maleimide-Activated Blue Carrier Protein enables conjugation of sulfhydryl-containing peptide antigens to this soluble immunogenic carrier protein for immunization and effective antibody production.
- Optimized activation—specifications require that each lot is activated with more than 400 moles of sulhydryl-reactive maleimide per mole of carrier, ensuring consistently high levels of conjugation
- Sulfhydryl-reactive—engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production
- Simple, rapid procedure—complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals
- High-quality carrier protein—Blue Carrier Protein is ultrapure Concholepas concholepas hemocyanin (CCH) prepared from molluscan hemolymph
- Highly immunogenic and soluble—CCH has a high molecular mass that elicits a stronger immune response than BSA or ovalbumin
Blue Carrier* Protein is a large hemocyanin that is highly immunogenic like keyhole limpet hemocyanin (KLH) yet is more soluble
Blue Carrier* Protein is specially purified hemocyanin from the mollusk Concholepas concholepas. The CCH protein is composed of two very large polypeptide subunits (404/351 kDa) that form an extremely stable heterodidecameric structure even in the absence of divalent cations. (By contrast, KLH has a less stable and soluble homodidecameric structure). The complex molecular arrangement of CCH subunits contains diverse repeated antigenic structures that elicit a strong immune reaction mediated by T and B lymphocytes.
Formulation: Activated protein lyophilized in 10 mM sodium phosphate, 150 mM sodium chloride, 10 mM EDTA, 50 mM sucrose, pH 7.2; For: 5 conjugation reactions, each with up to 2 mg sulfhydryl-containing peptide.
The carrier is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Numerous antigen molecules can be conjugated to each Blue Carrier Protein molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest.
Información para pedidos: Upon receipt store product at −20 °C. Product is shipped with an ice pack.
Precaución: For Research Use Only. Not for use in diagnostic procedures.
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