Pierce™ PNPP Substrates, Thermo Scientific

Supplier: LIFE TECHNOLOGIES MS

34045 34047 37620 37621
PI34045EA 237.51 USD
PI34045 PI34047 PI37620 PI37621
Pierce™ PNPP Substrates, Thermo Scientific
Assays ELISAs

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications

PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.


  • One-component alkaline phosphatase (AP) substrate
  • Absorbance maximum of 405 nm
  • Develops a yellow hue in solution upon reaction with AP
  • Safer to use—contains a non-mercury, non-azide preservative
  • Product is shipped at ambient temperature.


The substrate is offered in several formats:
PNPP Substrate
• Powder Form

PNPP Substrate Tablets
• 5 mg tablets with an inert filler as the balance

PNPP Substrate Kit
• PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.

1-Step™ PNPP Substrate Solution
• Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.


Store buffer at 4 °C and PNPP tablets at -20 °C.


Precaución: For Research Use Only. Not for use in diagnostic procedures.

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