Clean-Blot™ IP Detection Kit (HRP) contains enough Clean-Blot™ IP Detection Reagent and accessory reagents for 25 mini-blots or 2000 cm2 of membrane
- Bind and detect most species, IgG subclasses and isotypes of primary antibodies that are commonly used for Western blotting
- Effective with IP assays performed using Protein A, Protein G, or anti-IgG agarose beads and any blocking buffer
- Eliminates the extra work associated with covalently immobilizing IP antibodies as a means of overcoming Western blot interference
- Horseradish peroxidase (HRP) reagent for detection with chemiluminescent, fluorescent or colorimetric substrates
- Clear Western blot results for immmunoprecipitation assays without significant interference from denatured IgG bands
The Clean-Blot™ IP Reagent allows trouble-free Western blot detection of target proteins following IP assays (immunoprecipitation). It works by specifically binding to functional primary antibodies (whole IgG) without also binding to fragments of the IP antibodies, which usually accompany the immunoprecipitated protein through electrophoresis and membrane transfer. The Clean-Blot™ IP Reagent and Kit eliminate detection-interference from both heavy-chain (approx. 50 kDa) and light-chain (25 kDa) IgG-fragments of antibodies used for the initial immunoprecipitation assay.
Clean-Blot™ IP Detection Reagent is a horseradish peroxidase conjugate that is optimized for post-immunoprecipitation Western blot detection of primary antibodies without interference from denatured IP antibody fragments.
Información de suministro: Kit contains Clean-Blot™ Detection Reagent (HRP) (2.5 mL), StartingBlock T20 (TBS) Blocking Buffer (1 L), Pierce™ ECL Detection Reagent 1, Peroxide Solution (125 mL) and Pierce™ ECL Detection Reagent 2, Luminol Enhancer Solution (125 mL).
Precaución: Store at 4˚C. For Research Use Only. Not for use in diagnostic procedures.
