Pierce™ 660 nm Protein Assay, Thermo Scientific
Supplier: LIFE TECHNOLOGIES MS
Ready-to-use, detergent and reducing agent-compatible assay reagent to quickly measure (A660 nm) total protein concentration compared to a protein standard.
- Versatile to work with a range of detergents and reducing agents
- Fast and simple imple mix-and-read protocol for accurate protein quantitation
- Accurate standard curves that are more linear than with the Bradford assay method
- Flexible, assay may be performed in test tubes or microplates
- Requires conserve samples of 10 µL for microplate or 100 µL for the test tube procedures
- Convenient as room temperature storage is enough, no need for reagent equilibration before use
Assay is more linear than coomassie-based Bradford assays and compatible with higher concentrations of most detergents, reducing agents and other commonly used reagents
The Ionic Detergent Compatibility Reagent (IDCR) provides even broader detergent compatibility, making the 660 nm protein assay one of the only protein assays suitable for samples containing Laemmli SDS sample buffer with bromophenol blue and many common ionic detergents. IDCR completely dissolves by thorough mixing and does affect the sensitivity of the assay.
Although it produces a higher level of protein-to-protein variation (37%) than other assays, such as the BCA Protein Assay and its simpler single-reagent format and broader substance compatibility make the assay more convenient for many routine applications. For research use only, not for use in diagnostic procedures.
Información de suministro: Kit includes Pierce™ 660 nm Protein Assay Reagent (450 mL) and albumin standard pre-diluted set (7x3.5 mL). Pierce™ 660 nm Protein Assay Reagent is also available separately.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...