Aldehyde Dehydrogenase (from Yeast), MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

0219067925 0219067980 0219067983

IC19067925EA 112.12 USD

IC19067925 IC19067980 IC19067983

Aldehyde Dehydrogenase (from Yeast), MP Biomedicals

Enzymes

Aldehyde dehydrogenase belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor.

Presentation: Off-White Powder
Extinction Coefficient (E1%): 9.4 (279 nm)(Lit)
Optimum pH: 9.0
Gene ID: 855205
Uniport Number: P54114

Aldehyde dehydrogenase oxidizes a number of aliphatic and aromatic aldehydes. Acetyl-GSH is not hydrolyzed.

Aldehyde dehydrogenase is a tetramer and has several different isoforms. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate. Potassium ions and cysteine are essential for the enzyme′s activity. Rubidium or NH4+ can be substituted for K+, and glutathione for cysteine. Lithium, Na+, and Cs+ inhibit the reaction. Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme may be utilized to quantitate aldehydes present in blood.

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