β-Nicotinamide adenine dinucleotid, oxidised form (NAD, oxidised form) free acid ≥99%, powder

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

Synonyms: Nadide, NAD+, Nicotinamide adenine dinucleotide, oxidized

02160047.1 0216004701 0216004705
IC160047.1EA 52.87 USD
IC160047.1 IC16004701 IC16004705
β-Nicotinamide adenine dinucleotid, oxidised form (NAD, oxidised form) free acid ≥99%, powder
β-Nicotinamide adenine dinucleotid, oxidised form (NAD, oxidized form)

Soluble in water (1%).


β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.


Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.


Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.

Formula: C₂₁H₂₇N₇O₁₄P₂
MW: 663.43 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00036253
CAS Number: 53-84-9

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Specification Test Results

Extinction Coefficient EmM = 18.0 (260 nm, pH 7)(Lit.)

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