4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride), yellow powder
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Synonyms:
2-(4-Amidinophenyl)indole-6-carboxamidine Dihydrochloride, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride, DAPI.2HCl, 2-(4-Carbamimidoylphenyl)indole-6-carboximidamide Dihydrochloride, DAPI 2HCl
4',6-Diamidino-2-phenylindole Dihydrochloride is a cell permeable fluorescent minor groove-binding probe for DNA.
It binds to double-stranded DNA by forming a stable complex which fluoresce. DAPI selectively forms complexes with A-T, A-U and I-C base pairs (preferably A-T rich DNA), having one molecule of dye for every 3 base-pairs. DAPI in aqueous solution has two absorbance maxima, at 349 nm and at 263 nm. It can be used as a vital dye to stain nuclei in mature living pollen grains in conjuction with fluroescein diacetate.
Biochem/physiol Actions: Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Slightly soluble in water (10 mg/mL - clear, yellow to yellow-green solution - heat or sonication may be required).
4',6-Diamidino-2-phenylindole Dihydrochloride is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Formula:
C₁₆H₁₅N₅·2HCl MW: 350.25 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00012681 CAS Number: 28718-90-3 |
Specification Test Results
Identity Test | Passes |
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