4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride), yellow powder

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

Synonyms: 2-(4-Amidinophenyl)indole-6-carboxamidine Dihydrochloride, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride, DAPI.2HCl, 2-(4-Carbamimidoylphenyl)indole-6-carboximidamide Dihydrochloride, DAPI 2HCl

0215757401 0215757405 0215757410 0215757450
IC15757401EA 69.94 USD
IC15757401 IC15757405 IC15757410 76102-756
4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride), yellow powder
4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride)

4',6-Diamidino-2-phenylindole Dihydrochloride is a cell permeable fluorescent minor groove-binding probe for DNA.


It binds to double-stranded DNA by forming a stable complex which fluoresce. DAPI selectively forms complexes with A-T, A-U and I-C base pairs (preferably A-T rich DNA), having one molecule of dye for every 3 base-pairs. DAPI in aqueous solution has two absorbance maxima, at 349 nm and at 263 nm. It can be used as a vital dye to stain nuclei in mature living pollen grains in conjuction with fluroescein diacetate.


Biochem/physiol Actions: Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.


Slightly soluble in water (10 mg/mL - clear, yellow to yellow-green solution - heat or sonication may be required).


4',6-Diamidino-2-phenylindole Dihydrochloride is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Formula: C₁₆H₁₅N₅·2HCl
MW: 350.25 g/mol
Storage Temperature: Freezer
MDL Number: MFCD00012681
CAS Number: 28718-90-3
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