Protease (from S. aureas V8 strain), MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

0215197201 0215197205 0215197210

IC15197201EA 225.58 USD

IC15197201 IC15197205 IC15197210

Protease (from S. aureas V8 strain), MP Biomedicals

Enzymes

Protease from Staphylococcus aureus strain V8 is composed of a single polypeptide chain

Presentation: Off-white Powder
Extinction Coefficient (E1%): E1%280 = 4.26
pH: 4.0 and 7.8 with hemoglobin substrate (optimum pH)
Inhibitors: Diisopropyl fluorophosphate (DFP) and monovalent anions such as F-, Cl-, Br-, CH3COO- and NO3-.
Soluble in aqueous buffers (such as 10 mM Tris, pH 7.8 at 1 mg/mL).

Protease V8 is used for selective cleavage of proteins for amino acid sequence determination or peptide mapping.

The enzyme was isolated, by the method of Drapeau et al., from the culture filtrate of S. aureus V8 strain. The preparation is homogenous on ultracentrifugation and disc electrophoresis.

The Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl (COOH) terminal side of aspartic and glutamic acid residues.

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