Collagenase Type 3, MP Biomedicals
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
0215070401
0215070405
IC15070401EA
712.25
USD
IC15070401
IC15070405
Collagenase Type 3, MP Biomedicals
Enzymes
For typical assay, dissolve enzyme at a concentration of 1 mg/mL in 0.05 M TES with 0.36 mM calcium chloride, pH 7.5
One unit liberates one µmole of L-leucine equivalents from collagen in 5 hours at 37 °C, pH 7.5.
Dilutions run are 1/10 and 1/20 in the above buffer.
Activators: Ca2+ is required. ; Inhibitors: Metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by a2-macroglobulin.
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