beta-Galactosidase, MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

0215003901 0215003903

IC15003901EA 221.42 USD

IC15003901 IC15003903

beta-Galactosidase, MP Biomedicals

Enzymes

One unit catalyzes the hydrolysis of 1 µmole of o-nitrophenyl-β-D-galactopyranoside per minute at 25 °C, pH 7.5.

Substrate Specificity: The enzyme specifically hydrolyzes β-D-galactosyl linkage

Inhibitors: p-Chloromercuribenzoate, lodoacetamide, heavy metal ions (Zn++, Fe++, Zn++, Cd++, Cu++, Pb++, Ag+, Hg++), Ionic Detergents (SDS, DAC, etc.). Contaminants: The preparation is practically free from other glycosidases (a-galactosidase, a-,b-glucosidase, a-,b-mannosidase, etc.) and proteinase. Principle: o-Nitrophenyl-b-D-galactopyranoside (ONPG) b-galactosidase > o-Nitrophenol (ONP) + D-Galactose. The appearance of o-nitrophenol is measured at 410 nm by spectrophotemetry. Thermal Stability: Below 50 °C (pH 7.3, 15 min) (Lit.), Optimum Temperature: 50 - 55 °C (Lit.)

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

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