TRIS HCl (tris(hydroxymethyl)aminomethane hydrochloride) ≥99%, Reagent Grade
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.
Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid .
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
Formula:
NH₂C(CH₂OH)₃·HCl |
MDL Number:
MFCD00012590 CAS Number: 1185-53-1 |
Specification Test Results
Synonyms | Trometamol; Tromethamine; Tris-[hydroxymethyl]aminomethane; 2-Amino-2-(hydroxymethyl)-1,3-propanediol |
Form | White Crystalline Powder |
Storage | Store at Room Temperature (15 - 30 °C). |
pH | 4.0 - 5.0 (5% aq soln) |
pka | 8.08 (25 °C) |
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