2,3,5-Triphenyltetrazolium chloride (TTC), white powder

Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Synonyms: Tetrazolium salt, Tetrazolium Red, 2,3,5-Triphenyl-2H-tetrazol-3-ium chloride, 2,3,5-Triphenyl-2H-tetrazolium chloride, TTC

0210312610 0210312625 0210312650 0210312680 0210312601
IC10312610EA 93.84 USD
IC10312610 IC10312625 IC10312650 IC10312680 75875-602
2,3,5-Triphenyltetrazolium chloride (TTC), white powder
2,3,5-Triphenyltetrazolium chloride (TTC)

2,3,5-Triphenyltetrazolium Chloride is a redox indicator. In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains as white TTC in areas of necrosis since these enzymes have been either denatured or degraded.
2,3,5-Triphenyltetrazolium Chloride is commonly used in biochemical experiments especially to indicate cellular respiration. TTC has been employed in autopsy pathology to assist identification of post-mortem myocardial infarctions. Healthy viable heart muscle will stain deep red from the cardiac lactate dehydrogenase; while areas of potential infarctions will be more pale. Useful indicator for reducing substances


  • Presentation: White Powder
  • Soluble in ethanol, alcohol, water (10 mg/mL - clear, colorless solution) or acetone. Insoluble in ether.
  • Storage temperature: +4 °C, protect from light


2,3,5-Triphenyltetrazolium Chloride is a redox indicator. In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains as white TTC in areas of necrosis since these enzymes have been either denatured or degraded.

Formula: C₁₉H₁₅ClN₄
MW: 334.81 g/mol
Melting Pt: 243…250 °C (lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00011963
CAS Number: 298-96-4
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Specification Test Results

Identity Test Passes
FTIR Conforms to Standard
I max (water) 246 ± 5 nm

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