Sodium dodecyl sulfate (SDS) ≥99%, white powder
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Danger
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Synonyms:
Dodecyl sulphate sodium salt, Sodium dodecyl sulfate, Dodecyl sulphuric acid sodium salt, Lauryl sulphate sodium salt, Sodium lauryl sulphate
SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions.
- Auto Ignition: 248 °C (powder containing 90% SLS); 447 °C (solution containing 14.8%SLS) (Lit.)
- Vapor Pressure: Practically zero (Lit.)
- Aggregation Number: 62
- Critical Micelle Concentration (CMC): 8.27mM
- Soluble in water (200 mg/mL - clear, faint yellow solution), and ethanol (0.1 g/10 mL).
Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on protein size.
An anionic detergent typically used to solubilize and denature proteins for electrophoresis. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material.
SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on protein size. The critical micelle concentration (CMC) in pure water at 25 °C is 8.27 mM, and the aggregation number at this concentration is usually considered to be about 62. The micelle ionization fraction (α) is around 0.3 (or 30%).
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Formula:
CH₃(CH₂)₁₁OSO₃Na MW: 288.38 g/mol Melting Pt: 180…210 °C Density: Storage Temperature: Ambient |
MDL Number:
MFCD00036175 CAS Number: 151-21-3 Merck Index: 14,08636 |
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Specification Test Results
| Identity Test | Passes |
| Purity | ≥99% |
| FTIR | Conforms to Standard |
| OD260 (1% aq soln) | ≤0.1 |
| OD280 (1% aq soln) | ≤0.1 |
| pH (1% aq soln) | 6-7 |
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