Guanidinium hydrochloride, white powder, Practical grade

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

02101905.2 0210190501 0210190505
IC101905.2EA 55.27 USD
IC101905.2 IC10190501 IC10190505
Guanidinium hydrochloride, white powder, Practical grade
Guanidinium hydrochloride

Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins.


This strong denaturant can solubilise insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.


Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins.


Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.

Formula: NH₂C(=NH)NH₂·HCl
MDL Number: MFCD00013026
CAS Number: 50-01-1
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Specification Test Results

Identity Test Passes
Melting Point 175 - 190 °C
FTIR Conforms to Standard
Loss on Drying ≤1,0%
Solubility (6M aq soln) R Relatively Clear to Cloudy Yellow Solution with Sediment on Standing

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