5-Methylphenazinium methyl sulfate, dark yellow powder
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Warning
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Synonyms:
PMS, 5-Methylphenazin-5-ium methyl sulphate, Phenazine methosulphate, Phenazine methylsulphate
Phenazine Methosulfate is an electron acceptor and carrier in enzyme systems. The oxidized form is yellow and the reduced form is colorless.
Phenazine methosulfate is used in tyrosine transaminase test and for enzymic determination of ethanol in blood by the colorimetric micromethod. Used with ascorbic acid to determine nitric oxide reductase activity. Since the reduced PMS is easily oxidized by oxygen, it is used in assays as an electron carrier between enzymes and oxygen, cytochrome c, indophenols, or tetrazolium salts. The reduced PMS is used as an electron donor to reduce cytochrome c or in photosynthetic experiments. Usage of PMS for detection of specific dehydrogenases has been reported.
PMS is reduced by flavoproteins such as succinic dehydrogenase. PMS is reduced non-enzymatically by NADH and NADPH. PMS is also reduced by dithionite, sodium borohydride, ascorbic acid, reduced ubiquinones, and reduced vitamin K. PMS reacts with sulfhydryl groups including those of enzymes.
Soluble in water(800 mg/4 mL), ethanol,methanol (5% w/v); insoluble in benzene, diethyl ether.
PMS is reduced by flavoproteins such as succinic dehydrogenase. PMS is reduced non-enzymatically by NADH and NADPH. PMS is also reduced by dithionite, sodium borohydride, ascorbic acid, reduced ubiquinones, and reduced vitamin K. PMS reacts with sulfhydryl groups including those of enzymes.
Formula:
C₁₄H₁₄N₂O₄S MW: 306.34 g/mol |
MDL Number:
MFCD00011923 CAS Number: 299-11-6 EINECS: 206-072-1 |
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