LONGAMP® Hot Start Taq 2X Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)

LongAmp Hot Start Taq 2X Master Mix - 100 rxns

LongAmp Hot Start Taq 2X Master Mix - 100 rxns

M0533L M0533S

102500-066EA 846 USD

102500-066 102500-068

LONGAMP® Hot Start Taq 2X Master Mix, New England Biolabs

Nucleic Acid Reagents End-point PCR Enzymes and Kits

LongAmp Hot Start Taq 2X Master Mix contains a unique blend of aptamer-based Hot Start Taq and Deep VentR™ DNA Polymerases.

The aptamer-based inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. The LongAmp® Hot Start Taq 2X Master Mix does not require a separate high temperature incubation step to activate the enzyme. The 3´→5´ exonuclease activity of Deep VentR DNA Polymerase increases the fidelity and robust amplification of Hot Start Taq DNA Polymerase. LongAmp® Hot Start Taq DNA Polymerase offers two-fold higher fidelity than Hot Start Taq DNA Polymerase alone. The convenient master mix formulation is supplied at a 2X concentration and contains dNTPs and Mg++, requiring only the addition of primers and DNA template for robust amplification. A wide range of PCR products can be generated; up to 30 kb from lambda or human genomic DNA.

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