EcoRI-HF®, Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
The NEB EcoRI HF® is an E.coli strain that carries the cloned and modified EcoRI gene from E. coli RY13 (R.N. Yoshimori).
High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
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