Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH₂ Trifluoroacetate

Supplier: BACHEM AMERICAS INC

4030841.0001 4030841.0005
M-2105.0001BAEA 408.5 USD
M-2105.0001BA M-2105.0005BA
Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH₂ Trifluoroacetate
Proteins and Peptides

The FRET substrate Mca-RPKPYA-Nva-WMK(Dnp)-amide was hydrolyzed 60 times more rapidly by stromelysin 1 (MMP-3) (kcat/Km= 59400 M⁻¹s⁻¹) than by interstitial collagenase (MMP-1). However, it showed little discrimination between MMP-3, gelatinase A (MMP-2) (kcat/Km= 54000 M⁻¹s⁻¹), and gelatinase B (MMP-9) (kcat/Km= 55300 M⁻¹s⁻¹). Metalloelastase (MMP-12) digested this fluorogenic substrate with a kcat/Km of 243000 M⁻¹s⁻¹. For a fluorescent standard see M-2250.

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