Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH₂ Trifluoroacetate
Supplier: BACHEM AMERICAS INC
4026036.0001
4026036.0005
M-1895.0001BAEA
402.2
USD
M-1895.0001BA
M-1895.0005BA
Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH₂ Trifluoroacetate
Proteins and Peptides
Mca-PLGL-Dpa-AR-amide is a very sensitive FRET substrate for continuous assays and in situ determination of the matrix metalloproteinase activity. Cleavage at the Gly-Leu bond separates the highly fluorescent Mca group from the efficient 2,4-dinitrophenyl quencher, resulting in an increase in fluorescence intensity. The kcat/Km values for the punctuated metalloproteinase (MMP-7) and for gelatinase (MMP-2), e.g., are 1.7·10⁵ and 6.3·10⁵ M⁻¹s⁻¹, respectively.
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