Boc-Val-Pro-Arg-AMC
Supplier: BACHEM AMERICAS INC
Thrombin substrates are often cleaved as well by trypsin and trypsin-like enzymes, e.g. I-1365, I-1560, J-1120, and L-1150.
Boc-VPR-AMC, a specific, highly fluorogenic substrate for thrombin and the thrombin-staphylocoagulase complex. The thrombin assay has been used for the indirect determination of coagulation factor III. Trypsin, tryptase from rat mast cells, and two trypsin-like enzymes from sperm of the ascidian Halocynthia roretzi, acrosin and spermosin, also hydrolyze this substrate. Furthermore, Boc-VPR-AMC is cleaved by the Kex2 endoprotease (kexin, previously known as proteinase yscF), a membrane-bound, calcium-dependent serine protease from yeast α-cells.
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