MIDI FlexTubes, IBI Scientific

Supplier: IBI Scientific
IB48010 IB48020 IB48013 IB48023 IB48002 IB48005 IB48014 IB48024 IB48110 IB48112 IB48113 IB48114 IB48120 IB48121 IB48122 IB48123 IB48130 IB48131 IB48132 IB48133 IB48134 IB48135
16003-696PK 196.06 USD
16003-696 16003-698 83007-914 83007-918 89369-446 89369-452 89369-462 89369-468 89369-506 89369-508 89369-510 89369-512 89369-514 89369-516 89369-518 89369-520 89369-522 89369-524 89369-526 89369-528 89369-530 89369-532
MIDI FlexTubes, IBI Scientific
Tubes Dialysis Tubes
IBI MIDI FlexTubes are typically used for the extraction of proteins, RNA, DNA, or oligonucleotides (>20 nt) from polyacrylamide, agarose, or any other gel matrix in a running buffer.

MIDI FlexTubes require a minimum of 0.5 µg of protein at the start of extraction. The maximum size of the gel slice that can be inserted into the MIDI FlexTube is 1×0.5 cm.

The yield of DNA/RNA recovery from an agarose gel or polyacrylamide gel is 90%. The yield of protein recovery from an SDS-PAGE gel is 70%. The FlexTube membrane is ultra-clean, sulfur- and heavy metal-free, and EDTA treated.

They are also used for dialysis or buffer exchange of small volumes (50 to 800 µl), preparation of protein samples for MALDI-MS, 2D gel dots extraction, and sample concentrations. IBI MIDI FlexTubes are tested by using them in the extraction of proteins, RNA, or DNA fragments of various sizes from either agarose or polyacrylamide gels. IBI MIDI FlexTube kits are also tested for simple dialysis of salts or buffer exchanges. The quality of the isolated protein, RNA, or DNA fragments, or of the sample after dialysis, is checked by several assays commonly used for proteins, nucleic acids, and dialysis. These devices combine two modes of action, electro-elution of macromolecules from polyacrylamide or agarose gels and buffer exchange of small volume samples. These devices allow rapid run times and high performance in either mode, and extract the macromolecules without contamination.
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