You Searched For: Western Blotting Reagents

Application Notes Why You Should Leave the Darkroom Chemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your Experiment Digital imaging for Western Blots: Why it’s Essential and What to Look for in a Digital Imaging System Imaging...

High-sensitivity chemiluminescent and near IR applications for Western blotting with the Analytik Jena UVP ChemStudio series of lowlight imagers Webinar Thursday October 8 2020 2:00 pm – 3:00 pm EDT Replay Email Alerts Free Registration Chat Area ...

Advanced Imaging Systems The Azure 300-600 offer imaging capabilities for chemiluminescence UV RGB NIR blue light and white light depending on the model that fits your needs. The 600 offers the most flexible imaging options but each model can be u...

Three Types of Antibodies for Western Blot Types of antibodies for western blot In general various types of antibodies for western blot are used to detect and visualize specific proteins of interest. The primary antibodies are the key components t...

Azure Portfolio Overview - How Innovation Drives Performance. Webinar Tuesday 6th October 2020 14:00 to 15:00 EST Replay Email Alerts Free Registration Chat Area Certificate of attendance We build innovative high-performance imagers and reagents d...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Fluorescent Western blots are the gold standard for quantitative Westerns. They are ideal for detecting multiple proteins simultaneously (multi-plexing) allowing in-lane normalization and detection of your protein of interest and loading control a...

Blocking Buffer Azure Fluorescent Blot Blocking Buffer Ready-to-use block that stabilizes fluorescent signal Azure Fluorescent Blot Blocking Buffer is optimized for use with Azure’s fluorescent Western blotting systems. The 1x solution is ready-to...

Introduction Change is hard. It takes work to adapt to a new technology and to get used to something different. It’s especially hard if you have something that already seems to work. This is a big reason people still use film and darkrooms to proc...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

Fluorescent Demo Kit A demo kit is a great way to try out fluorescent Western blotting in your own lab with your own samples and primary antibodies. It the supplies you need to perform single color fluorescent Western blotting: PVDF Membrane Fluor...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

ECL Substrate Which Azure substrate is right for you? Azure Substrate Radiance™ Substrate Radiance™ Plus Substrate Use Extended dynamic range for detecting low and high abundance proteins within the same blot Low abundance protein detection Sensit...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Azure cSeries Advanced Imaging Systems The c300-c600 offer imaging capabilities for chemiluminescence UV RGB NIR blue light and white light depending on the model that fits your needs. The c600 offers the most flexible imaging options but each mod...