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Proteomics Tools and Equipment Protein Expression is the process of generating a specific protein by manipulating the gene expression in a living organism. The organisms utilized for protein expression must be easy to culture and maintain grow rap...

Azure Biosystems is dedicated to designing instruments that can deliver industry-leading performance across a broad range of laboratory applications without overcomplicating the user experience. We have designed a suite of upgradeable cSeries inst...

What is western blotting? Overview of western blotting Western blotting is the technique for identifying proteins of interest in a sample using gel electrophoresis to separate sample proteins. This technique has paved the way in biomedical science...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Western blotting antibody selection and detection methods Introduction to western blotting Western blotting is one of the most accurate protein detection methods used in countless medical and research applications. This complex laboratory techniqu...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

Protein Electrophoresis and Western Blotting Getting accurate and high-quality results depends on having the supplies and equipment you need to do your job efficiently and effectively. That’s why Avantor® carries your preferred products from brand...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Azure Biosystems Western Blotting Azure Biosystems offers top-of-the-line reagents and imaging systems for an efficient Western blotting workflow. With unique and flexible imaging systems supported by a range of reagents and supplies Azure can get...

Azure Biosystem – Western blotting Reagent Samples ECL Substrate Our ECL substrates for chemiluminescent detection provide sensitivity and quantitative ability Sample today! Blocking Buffer Our blocking buffers are formulated to reduce background ...

Introduction Change is hard. It takes work to adapt to a new technology and to get used to something different. It’s especially hard if you have something that already seems to work. This is a big reason people still use film and darkrooms to proc...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Three Types of Antibodies for Western Blot Types of antibodies for western blot In general various types of antibodies for western blot are used to detect and visualize specific proteins of interest. The primary antibodies are the key components t...

Fluorescent Western blots are the gold standard for quantitative Westerns. They are ideal for detecting multiple proteins simultaneously (multi-plexing) allowing in-lane normalization and detection of your protein of interest and loading control a...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...