You Searched For: Proteins and Peptides

Proteomics Tools and Equipment Protein Expression is the process of generating a specific protein by manipulating the gene expression in a living organism. The organisms utilized for protein expression must be easy to culture and maintain grow rap...

Protein Expression Technology Kits and Resources Protein expression is the process of generating a specific protein by manipulating the gene expression in a living organism. The organisms used for protein expression technology must be easy to cult...

Introduction to SDS-PAGE – Separating Proteins Based on Molecular Weight Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis or SDS-PAGE is a widely used technique for separating proteins based on their molecular weight. In this technique pr...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

Monoclonal Antibodies: Understanding Their Production and Clinical Applications Monoclonal antibodies mAbs have transformed the field of medicine thanks to their remarkable specificity and effectiveness in treating various diseases. This article w...

Protein Electrophoresis and Western Blotting Getting accurate and high-quality results depends on having the supplies and equipment you need to do your job efficiently and effectively. That’s why Avantor® carries your preferred products from brand...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

Antibody and Protein and Production in Suspension CHO and HEK Cells The shift in therapeutics towards biologics has created a need for mammalian systems that can quickly produce high titer proteins in a consistent reproducible manner. Transient tr...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Avantor® Biopharma Educational Material Learn all you need to know on biopharma its R&D and manufacturing processes and technologies advancing patient treatments and therapies. What Is Buffer Management Uncover the significance of effective buffer...

What is western blotting? Overview of western blotting Western blotting is the technique for identifying proteins of interest in a sample using gel electrophoresis to separate sample proteins. This technique has paved the way in biomedical science...

The Function of mRNA in Biopharma Messenger RNA (mRNA) plays a critical role in gene expression a process in which stored genetic information in DNA is used to create proteins. This RNA molecule acts as an intermediary between the DNA and ribosome...

GE Healthcare Life Sciences Workflow for Better Science GE Healthcare Life Sciences provide the expertise and tools for basic research of cells and biomolecules as well as tools for protein purification drug discovery and cell therapy research. Le...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...