You Searched For: Protein Assays

What Is Protein Electrophoresis Protein electrophoresis is a widely used technique in biochemistry and molecular biology that helps separate and analyze proteins based on their charge and size. The process involves applying an electric field to a ...

What Is Protein Aggregation? Protein aggregation is the phenomenon where proteins and biomolecules come together to form clusters or aggregates. These aggregates can manifest in various forms such as soluble and insoluble reversible and irreversib...

Strategies for Efficient Recombinant Protein Expression Recombinant protein expressions are essential in biotechnology and biomedical research. Recombinant proteins which are proteins produced through genetic engineering techniques have numerous a...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

An Overview of Recombinant Protein Production Recombinant protein production is a fundamental technique in biotechnology and molecular biology that involves crafting proteins not typically found in an organism. This is done by introducing specific...

Microbial based recombinant proteins A wide range of recombinant proteins is produced using microbial (E. coli or yeast) fermentation – often being the preferred production method to drive efficiencies in the production process and to be faster to...

Introduction to SDS-PAGE – Separating Proteins Based on Molecular Weight Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis or SDS-PAGE is a widely used technique for separating proteins based on their molecular weight. In this technique pr...

Protein Expression Technology Kits and Resources Protein expression is the process of generating a specific protein by manipulating the gene expression in a living organism. The organisms used for protein expression technology must be easy to cult...

Overview of Affinity Chromatography Affinity chromatography involves the binding affinity between the target protein and a ligand immobilized on a stationary phase within a chromatography column. One of the advantages of affinity chromatography is...

Pathogen Detection with the BAX® System X5 The BAX® System X5 PCR Assay for genus Listeria detects the target organism from standard enrichments of a variety of food and environmental samples. The method includes automated sample preparation in th...

Protein Electrophoresis and Western Blotting Getting accurate and high-quality results depends on having the supplies and equipment you need to do your job efficiently and effectively. That’s why Avantor® carries your preferred products from brand...

Protein Sample Preparation for Mass Spectrometry Protein sample preparation In order to run a successful mass spectrometry analysis you will need to properly prepare your sample. This includes a number of steps that may be specialized depending on...

The Role of RNA in Protein Synthesis Ribonucleic acid (RNA) plays a crucial role in protein synthesis. Protein synthesis occurs in two main stages: transcription and translation. During transcription the DNA sequence of a gene is used as a templat...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

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Antibody and Protein and Production in Suspension CHO and HEK Cells The shift in therapeutics towards biologics has created a need for mammalian systems that can quickly produce high titer proteins in a consistent reproducible manner. Transient tr...