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Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Sterilizing Fittings: The Critical Factors Consider several important factors when choosing a fitting for an application: the size and connection style chemical compatibility and certification/approval requirements. These factors alone can be chal...

What Is Protein Aggregation? Protein aggregation is the phenomenon where proteins and biomolecules come together to form clusters or aggregates. These aggregates can manifest in various forms such as soluble and insoluble reversible and irreversib...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

An Overview of Recombinant Protein Production Recombinant protein production is a fundamental technique in biotechnology and molecular biology that involves crafting proteins not typically found in an organism. This is done by introducing specific...

An Overview of Proteomics Proteomics is the study of proteins in a biological system that offers the required understanding of an organism’s structure and function compared to genomics. Proteins are the functional units of cells performing various...

Chemical Permeation Guide What is permeation? Permeation is the process by which a potentially hazardous chemical moves through a material on a molecular level. Molecules of chemical adsorb onto the outer surface of the material. They then enter a...

An Overview of Protein Expression Systems Protein expression systems play a vital role in facilitating essential biological functions. This article highlights the significance of these systems and their contribution to the expression of recombinan...