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Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

33 LC Colum n Selection – C18 Reversed-Phase M aterials www.hichrom.co.uk • sales@hichrom.co.uk • Tel: +44 (0) 118 930 3660 Hichrom Lim ited C18 Reversed-Phase Materials SPECIFICATIONS OF C18 BONDED RP MATERIALS Octadecyl (ODS) or C18 bonded phase...