You Searched For: Blotting Kits

High-sensitivity chemiluminescent and near IR applications for Western blotting with the Analytik Jena UVP ChemStudio series of lowlight imagers Webinar Thursday October 8 2020 2:00 pm – 3:00 pm EDT Replay Email Alerts Free Registration Chat Area ...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

Avantor® Life Sciences Educational Material Access Avantor’s expert life sciences insights with our comprehensive reference and educational material. Three Types of Antibodies for Western Blot The antibodies used to detect the target protein in a ...

Blocking Buffer Azure Fluorescent Blot Blocking Buffer Ready-to-use block that stabilizes fluorescent signal Azure Fluorescent Blot Blocking Buffer is optimized for use with Azure’s fluorescent Western blotting systems. The 1x solution is ready-to...

Buffers Buffers Azure Protein Free Blot Blocking Buffer Azure Blot Washing Buffer Azure Fluorescent Wash Buffer Azure Transfer Buffer Azure Fluorescent Blot Blocking Buffer

Application Notes Why You Should Leave the Darkroom Chemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your Experiment Digital imaging for Western Blots: Why it’s Essential and What to Look for in a Digital Imaging System Imaging...

Advanced Imaging Systems The Azure 300-600 offer imaging capabilities for chemiluminescence UV RGB NIR blue light and white light depending on the model that fits your needs. The 600 offers the most flexible imaging options but each model can be u...

Fluorescent Western blots are the gold standard for quantitative Westerns. They are ideal for detecting multiple proteins simultaneously (multi-plexing) allowing in-lane normalization and detection of your protein of interest and loading control a...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

Three Types of Antibodies for Western Blot Types of antibodies for western blot In general various types of antibodies for western blot are used to detect and visualize specific proteins of interest. The primary antibodies are the key components t...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

Introduction Change is hard. It takes work to adapt to a new technology and to get used to something different. It’s especially hard if you have something that already seems to work. This is a big reason people still use film and darkrooms to proc...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

ECL Substrate Which Azure substrate is right for you? Azure Substrate Radiance™ Substrate Radiance™ Plus Substrate Use Extended dynamic range for detecting low and high abundance proteins within the same blot Low abundance protein detection Sensit...

Azure Biosystems Western Blotting Azure Biosystems offers top-of-the-line reagents and imaging systems for an efficient Western blotting workflow. With unique and flexible imaging systems supported by a range of reagents and supplies Azure can get...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...