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Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

Using Type 1 Water (18.2 MΩ-cm) for High Performance Liquid Chromatography in the Food & Beverage Industry Tammy Goodman Commercial Manager Water Purification Thermo Scientific High Performance Liquid Chromatography (HPLC) is widely used in the fo...

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At Mirus Bio we know it’s all about expression. Introducing the new CHOgro® Expression System a transient transfection platform that finally gets high protein titers with robust cell growth in the most relevant CHO cells. • Efficient – Enables hig...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

Azure Biosystems is dedicated to designing instruments that can deliver industry-leading performance across a broad range of laboratory applications without overcomplicating the user experience. We have designed a suite of upgradeable cSeries inst...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

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