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Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Refrigerant Temperature Recommended Use For Other Considerations Dry Ice -78°C(-109°F) Products which must be kept frozen for extended periods of time Dry ice is regulated as a hazardous material in air transport and should not be used with produc...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

PRODUCT CHECKLIST ☐ Cell Biology Antibodies Cell Culture Equipment Cellular Assays and Signal Transduction Extracellular Matrices and Coated Plates Immunoassays Media Classical Mycoplasma Detection and Removal Serum Serum-Free Specialty Supplement...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

Maintaining Sample Integrity with Aerosol-blocking Tips Liquid Handling Consumables Thermo Fisher Scientific Because of the sensitivity of PCR a variety of factors can compromise the integrity of the reaction. This illustrates the importance of us...

LABORATORY CHECKLIST Chromatography ☐ AA Accessories ☐ Bio-Chromatography ☐ Buffers ☐ Bulk Media Alumina Ion-Exchange Irregular and Spherical Silica Normal Phase Reversed Phase ☐ CE and CE/MS Capillaries ☐ Flash Chromatography Columns Empty Glass ...

Fluorescent Western blots are the gold standard for quantitative Westerns. They are ideal for detecting multiple proteins simultaneously (multi-plexing) allowing in-lane normalization and detection of your protein of interest and loading control a...

33 LC Colum n Selection – C18 Reversed-Phase M aterials www.hichrom.co.uk • sales@hichrom.co.uk • Tel: +44 (0) 118 930 3660 Hichrom Lim ited C18 Reversed-Phase Materials SPECIFICATIONS OF C18 BONDED RP MATERIALS Octadecyl (ODS) or C18 bonded phase...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

TECHNICAL NOTE E1-ClipTip multichannel pipettes The E1-ClipTip Equalizer electronic pipette and Multidrop Combi Reagent Dispensers help improve the efficiency of preparing samples for RT-PCR and effort in these steps the Multidrop Combi Reagent Di...

Azure Biosystems is dedicated to designing instruments that can deliver industry-leading performance across a broad range of laboratory applications without overcomplicating the user experience. We have designed a suite of upgradeable cSeries inst...

Avantor Seradigm Product Information Bovine serum: Product and intended use Fetal Bovine Serum (FBS} FB Essence and Bovine Calf Serum (BCS) are commonly used growth supplements in cell culture. They are derived from the blood of source animals. Av...