You Searched For: Transfer+Membranes

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

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Syringe Filter Efficiency and Effect of Filtration on HPLC Column Life Syringe Filter Efficiency and Effect of Filtration on HPLC Column Life Introduction Background on Column Failure Test Methods Test Results and Discussion Function of Filtration...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

Fluorescent Western blots are the gold standard for quantitative Westerns. They are ideal for detecting multiple proteins simultaneously (multi-plexing) allowing in-lane normalization and detection of your protein of interest and loading control a...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

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CHALLENGE A top 25 biopharmaceutical company was using various external contract research organizations (CROs) to conduct GLP studies in order to support product development and new drug candidate submissions. After over 15 years of conducting the...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

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TECHNICAL NOTE E1-ClipTip multichannel pipettes The E1-ClipTip Equalizer electronic pipette and Multidrop Combi Reagent Dispensers help improve the efficiency of preparing samples for RT-PCR and effort in these steps the Multidrop Combi Reagent Di...

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