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The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

MEDIA AND BUFFER PREPARATION SERVICES ACCELERATE SCIENTIFIC RESEARCH Problem During a Lean Six Sigma business process assessment at one of the world’s largest pharmaceutical companies, VWRCATALYST found that scientists were spending substantial ti...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

LABORATORY CHECKLIST Clinical ☐ Blood Bank Blood Typing Tubes Prelabeled Centrifuges Copper Sulfate Solutions Crossmatch Racks Dilution Tubes Disposable Culture Tubes Disposable Pasteur Pipets Disposable Transfer Pipets Dry Bath/Block Heaters Exam...

MycoAlert-Inst-Control_Set_TS-LT07-518-3 Page | 1 of 2 Lonza Walkersville Inc. Walkersville MD 21793-0127 USA U.S. Scientific Support: 800 521 0390 scientific.support@lonza.com EU/ROW Scientific Support: +32 87 321 611 scientific.support.eu@lonza....

LABORATORY CHECKLIST Clinical ☐ Blood Bank Blood Typing Tubes Prelabeled Centrifuges Copper Sulfate Solutions Crossmatch Racks Dilution Tubes Disposable Culture Tubes Disposable Pasteur Pipets Disposable Transfer Pipets Dry Bath/Block Heaters Exam...

Cell culture ☐ Animal and Human Sera Fetal Bovine Serum (FBS) FBS Alternatives Bovine Serum Albumin (BSA) Other Sera ☐ Classical Media DMEM IMDM F-12 Leibovitz’s L-15 MCDB 131 MEM RPMI ☐ Serum-Free and Specialty Media CHO Insect Serum-Free (Genera...

MycoAlertPLUS-Inst-General-Inst-LT07-701 Page | 1 of 8 Lonza Walkersville Inc. Walkersville MD 21793-0127 USA U.S. Scientific Support: 800 521 0390 scientific.support@lonza.com EU/ROW Scientific Support: +32 87 321 611 scientific.support.eu@lonza....

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Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

MycoAlert-Inst-General_INST-LT07-118 All trademarks herein are marks of Lonza Group or its subsidiaries. Developed by Lonza Nottingham Ltd. Page 1 of 7 www.lonza.com U.S. Scientific Support: 800-521-0390 scientific.support@lonza.com EU/ROW Scienti...

MycoAlert-Inst-General_INST-LT07-118 All trademarks herein are marks of Lonza Group or its subsidiaries. Developed by Lonza Nottingham Ltd. Page 1 of 7 www.lonza.com U.S. Scientific Support: 800-521-0390 scientific.support@lonza.com EU/ROW Scienti...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

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Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

TITRATION THEORY AND PRACTICE 2 TITRATION THEORY AND PRACTICE The aim of this booklet is to explore titration from a historical theoretical and practical point of view dealing first with preset end-point titration then with inflection-point titrat...