You Searched For: Protein Purification Reagents

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Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

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LABORATORY CHECKLIST Clinical ☐ Blood Bank Blood Typing Tubes Prelabeled Centrifuges Copper Sulfate Solutions Crossmatch Racks Dilution Tubes Disposable Culture Tubes Disposable Pasteur Pipets Disposable Transfer Pipets Dry Bath/Block Heaters Exam...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

At Mirus Bio we know it’s all about expression. Introducing the new CHOgro® Expression System a transient transfection platform that finally gets high protein titers with robust cell growth in the most relevant CHO cells. • Efficient – Enables hig...

Quick Reference Protocol (QRP) Optimizing Plasmid DNA Delivery with TransIT® Transfection Reagents Quick Reference Protocol for DNA Transfection Optimization in a 12-well Plate Format OPTIMIZATION PROTOCOL FOR PLASMID DNA DELIVERY The following pr...

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TECHNICAL BULLETIN “Pipetting error” is a phrase that is commonly heard in research laboratories. When preparing reagent cocktails pipetting error is accommodated for. What is not typically discussed or published are the causes and ramifications o...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

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Using Type 1 Water (18.2 MΩ-cm) for High Performance Liquid Chromatography in the Food & Beverage Industry Tammy Goodman Commercial Manager Water Purification Thermo Scientific High Performance Liquid Chromatography (HPLC) is widely used in the fo...

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33 LC Colum n Selection – C18 Reversed-Phase M aterials www.hichrom.co.uk • sales@hichrom.co.uk • Tel: +44 (0) 118 930 3660 Hichrom Lim ited C18 Reversed-Phase Materials SPECIFICATIONS OF C18 BONDED RP MATERIALS Octadecyl (ODS) or C18 bonded phase...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...