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Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

Quick Reference Protocol (QRP) Optimizing Plasmid DNA Delivery with TransIT® Transfection Reagents Quick Reference Protocol for DNA Transfection Optimization in a 12-well Plate Format OPTIMIZATION PROTOCOL FOR PLASMID DNA DELIVERY The following pr...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

Introduction Change is hard. It takes work to adapt to a new technology and to get used to something different. It’s especially hard if you have something that already seems to work. This is a big reason people still use film and darkrooms to proc...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

At Mirus Bio we know it’s all about expression. Introducing the new CHOgro® Expression System a transient transfection platform that finally gets high protein titers with robust cell growth in the most relevant CHO cells. • Efficient – Enables hig...

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Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Ward’s Science Tel: (866) 260-0501 57 5 The degree of flammability of liquids or solids is numerically expressed by “flash point” (also and more accurately called “lower flash point”). The smaller the flash point number (expressed in degrees Fahre...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

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1 All About Elements: Sodium http://wardsci.com https://wardsci.com/store wardsci.com | 800-962-2660 2 Ward’s All About Elements Series Building Real-World Connections to the Building Blocks of Chemistry The periodic table of elements is an essent...

TECHNICAL BULLETIN “Pipetting error” is a phrase that is commonly heard in research laboratories. When preparing reagent cocktails pipetting error is accommodated for. What is not typically discussed or published are the causes and ramifications o...

1 All About Elements: Hydrogen https://wardsci.com/store wardsci.com | 800-962-2660 2 3 Fun Facts About… Hydrogen 1. Robert Boyle produced hydrogen gas as early as 1671 while he was experimenting with iron and acids. 2. Under the extreme pressures...