You Searched For: Membrane Dispensers

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Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Syringe Filter Efficiency and Effect of Filtration on HPLC Column Life Syringe Filter Efficiency and Effect of Filtration on HPLC Column Life Introduction Background on Column Failure Test Methods Test Results and Discussion Function of Filtration...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

Fluorescent Western blots are the gold standard for quantitative Westerns. They are ideal for detecting multiple proteins simultaneously (multi-plexing) allowing in-lane normalization and detection of your protein of interest and loading control a...

TECHNICAL NOTE E1-ClipTip multichannel pipettes The E1-ClipTip Equalizer electronic pipette and Multidrop Combi Reagent Dispensers help improve the efficiency of preparing samples for RT-PCR and effort in these steps the Multidrop Combi Reagent Di...

TECHNICAL NOTE E1-ClipTip multichannel pipettes The E1-ClipTip Equalizer electronic pipette and Multidrop Combi Reagent Dispensers help improve the efficiency of preparing samples for RT-PCR and effort in these steps the Multidrop Combi Reagent Di...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

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Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

wardsci.com 800.962.2660 Developing a purchasing plan and rigorously following the plan when ordering the school’s chemical supply will help safety prevent waste and save money. The following may help your school make chemical purchases efficient ...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

TECHNICAL BULLETIN “Pipetting error” is a phrase that is commonly heard in research laboratories. When preparing reagents and master mixes pipetting error is accommodated for. What is not typically discussed or published are the causes and ramific...

TECHNICAL BULLETIN “Pipetting error” is a phrase that is commonly heard in research laboratories. When preparing reagent cocktails pipetting error is accommodated for. What is not typically discussed or published are the causes and ramifications o...