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Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Refrigerant Temperature Recommended Use For Other Considerations Dry Ice -78°C(-109°F) Products which must be kept frozen for extended periods of time Dry ice is regulated as a hazardous material in air transport and should not be used with produc...

Bad reactions to latex gloves? PROTECT YOURSELF Protect yourself from Type I allergies. Introduction Occasionally wearing glove products can cause issues with the health of our skin. This predominantly manifests itself in the form of skin allergie...

Color-Coded Storage of Chemicals +ward’sscience 800 962-2660 • wardsci.com OSHA Accepted Industry Standard Labeling System AREA 1 Low Hazard Storage Code: Green Suitable for general storage area. AREA 2 Flammables Storage Code: Red Store in flamma...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

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The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

Chemicals and other sterilization methods can affect the strength flexibility surface appearance color dimensions or weight various plastics differently. These changes can include: Sterilizing your reusable plastic labware Stress on the polymer ch...

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