You Searched For: Expression Systems

At Mirus Bio we know it’s all about expression. Introducing the new CHOgro® Expression System a transient transfection platform that finally gets high protein titers with robust cell growth in the most relevant CHO cells. • Efficient – Enables hig...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

Quick Reference Protocol (QRP) Optimizing Plasmid DNA Delivery with TransIT® Transfection Reagents Quick Reference Protocol for DNA Transfection Optimization in a 12-well Plate Format OPTIMIZATION PROTOCOL FOR PLASMID DNA DELIVERY The following pr...

Ward’s Science Tel: (866) 260-0501 57 5 The degree of flammability of liquids or solids is numerically expressed by “flash point” (also and more accurately called “lower flash point”). The smaller the flash point number (expressed in degrees Fahre...

Manufacturing CUSTOM CHEMICALS AND SERVICES SUPPORTING SCIENTIFIC ADVANCES FOR HUMAN HEALTH VWR Custom Manufacturing Services2 VWR enables the advancement of science by providing high-quality chemicals and services customized to your product or ma...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Introduction Change is hard. It takes work to adapt to a new technology and to get used to something different. It’s especially hard if you have something that already seems to work. This is a big reason people still use film and darkrooms to proc...

Ward’s Science Tel: (866) 260-0501 2 Ward’s Science Tel: (866) 260-0501 3                            Ward’s Science Tel: (866) 260-0501 4                        Ward’s Science Tel: (866) 260-0501 5...

1 All About Elements: Beryllium http://wardsci.com https://wardsci.com/store wardsci.com | 800-962-2660 2 3 Fun Facts About… Beryllium 1. Prior to being named beryllium this element was known as glucinium which originated from the Greek word glyky...

1 All About Elements: Beryllium http://wardsci.com https://wardsci.com/store wardsci.com | 800-962-2660 2 3 Fun Facts About… Beryllium 1. Prior to being named beryllium this element was known as glucinium which originated from the Greek word glyky...

1 All About Elements: Helium https://wardsci.com/store wardsci.com | 800-962-2660 2 3 Fun Facts About…Helium 1. Helium is the second most abundant ele- ment after hydrogen in the universe and solar system. Approximately 11.3% of all atoms in the u...

TITRATION THEORY AND PRACTICE 2 TITRATION THEORY AND PRACTICE The aim of this booklet is to explore titration from a historical theoretical and practical point of view dealing first with preset end-point titration then with inflection-point titrat...