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Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

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Introduction Change is hard. It takes work to adapt to a new technology and to get used to something different. It’s especially hard if you have something that already seems to work. This is a big reason people still use film and darkrooms to proc...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Maintaining Sample Integrity with Aerosol-blocking Tips Liquid Handling Consumables Thermo Fisher Scientific Because of the sensitivity of PCR a variety of factors can compromise the integrity of the reaction. This illustrates the importance of us...

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Fluorescent Western blots are the gold standard for quantitative Westerns. They are ideal for detecting multiple proteins simultaneously (multi-plexing) allowing in-lane normalization and detection of your protein of interest and loading control a...

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Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

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BIOTIX COBRA PIPETTE USER’SGUIDE BIOTIX COBRA PIPETTE 2 Contents Page 1- INTRODUCTION 3 2- PARTS CHECK LIST 3 3- DESCRIPTION 4 4- SETTING THE VOLUME 6 5- USER ADJUSTMENT 7 6- PIPETTING 8 7- GENERAL GUIDELINES FOR GOOD PIPETTING 10 8- ACCESSORIES 1...

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Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

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