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Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...

1 Determination of Pesticide Residues in Red Wine Using a QuEChERS Sample Preparation Approach and LC-MS/MS Detection Mike Oliver Thermo Scientific Runcorn UK This application presents a fast easy and cost-effective method for the determination of...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...