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You Searched For: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-82021-274

Perfect-FOCUS™ Electrophoresis Clean-Up Kit, G-Biosciences

Supplier: G-Biosciences
Description: Perfect-FOCUS™ is designed to clean and concentrate protein samples that give poor protein spot resolution during 2D electrophoresis

Product Image-490005-012

Advansta WesternBright™ Sirius HRP Substrate Kits

Supplier: Advansta
Description: WesternBright Sirius is the most sensitive chemiluminescent HRP substrate offered by Advansta, specially engineered to produce an extremely strong signal to detect low-abundance proteins and to work with very low antibody concentrations. The substrate also produces a strong chemifluorescent signal that can be detected with fluorescence imagers.

Image Unavailable-95059-150

4CN Membrane Peroxidase Substrate, KPL

Supplier: KPL
Description: 4CN (4-chloro-1-napthol) is a reliable substrate for detection of membrane-bound proteins

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Product Image-102096-302

Synergel™ Agarose Additive, Electron Microscopy Sciences

Catalog Number: (102096-302)
Supplier: Electron Microscopy Sciences
Description: Improving Agarose Gel Performance
Product Image-PI34012

Pierce 1-Step™ Chloronaphthol Substrate Solution, Thermo Scientific

Catalog Number: (PI34012)
Supplier: Invitrogen
Description: The 1-Step™ chloronaphthol solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

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Product Image-PI80196

Pierce™ Substrate for Western Blotting, ECL Plus, Thermo Scientific

Catalog Number: (PI80196)
Supplier: Invitrogen
Description: The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

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Image Unavailable-101396-882

BioFX® Chemiluminescent Ultra Sensitive AP Microwell and/or Membrane Substrate (450 nm), Surmodics

Catalog Number: (101396-882)
Supplier: Surmodics
Description: Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and membrane assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the dioxetane substrate, antibody/antigen titers and blockers. Surmodics provides multiple 1-component AP chemiluminescent substrates with various intensities and wavelength emissions to allow the assay developer to choose the appropropriate substrate for the application.
Product Image-PI37552

Pierce™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent, Thermo Scientific

Supplier: Invitrogen
Description: Thermo Scientific Guardian Peroxidase Conjugate Stabilizer/Diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long-term storage.

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Product Image-PI34070

Pierce 1-Step™ NBT/BCIP plus Suppressor Substrate Solution, Thermo Scientific

Catalog Number: (PI34070)
Supplier: Invitrogen
Description: The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

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Product Image-PI62299

Restore™ Fluorescent Western Blot Stripping Buffer, Thermo Scientific

Supplier: Invitrogen
Description: The Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

Certificates

Product Image-PI20158

Pierce™ LightShift™ Chemiluminescent RNA EMSA Kit, Thermo Scientific

Catalog Number: (PI20158)
Supplier: Invitrogen
Description: The Thermo Scientific LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

SDS


Image Unavailable-100359-144

BioFX® Chemiluminescent Ultra Sensitive HRP Microwell Substrate, Surmodics IVD, Inc.

Catalog Number: (100359-144)
Supplier: Surmodics
Description: Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in Western Blot assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the luminol substrate, antibody/antigen titers and blockers.
Image Unavailable-100359-204

BioFX® Chemiluminescent Ultra Sensitive HRP Membrane Substrate, Surmodics IVD, Inc.

Catalog Number: (100359-204)
Supplier: Surmodics
Description: BioFX® Chemiluminescent Ultra Sensitive HRP Membrane Substrate is a luminol based formulation for western blot applications.
Product Image-101098-452

Alkaline Phosphatase (APP) Chromogen Substrates for IHC, Vector Laboratories

Supplier: Vector Laboratories
Description: Vector Laboratories alkaline phosphatase (AP) substrate options include standard substrates and ImmPACT substrates with higher sensitivity. The range of vibrant colors facilitates multiplex staining and improved contrast with pigmented tissues and counterstains.

Image Unavailable-PI20148E

Pierce™ LightShift™ Poly (dI-dC), Thermo Scientific

Catalog Number: (PI20148E)
Supplier: Invitrogen
Description: LightShift poly(dI-dC) can be used as competitor for nonspecific DNA binding proteins.

Certificates


Product Image-PI37574

Pierce™ 1-Step™ Ultra TMB-Blotting Solution, Thermo Scientific

Catalog Number: (PI37574)
Supplier: Invitrogen
Description: Thermo Scientific 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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