You Searched For: Protein Purification

Catalog Number: (95017-030)

Supplier: Cytiva

Description: NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

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Catalog Number: (TCP2461-1VIAL)

Supplier: TCI America

Description: Protein A is a type I membrane protein produced by several strains of Staphylococcus aureus. It has high-affinity binding sites that enable the purification of IgG obtained from various species such as humans, rabbit, mouse, and bovine. Protein A Agarose is prepared using a covalent coupling method that maintains the stability and binding characteristics of the product.
By using our resin, human IgGs can be eluted under milder conditions (such as at pH 4.0) as compared to elution by conventional methods.
Additional information is available on our website.

New Product

Catalog Number: (TCR0238-1VIAL)

Supplier: TCI America

Description: The rSRL is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. This lectin preferably binds to [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] glycan epitopes by its two distinct carbohydrate-binding sites.
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rSRL-LecBeads (1 ml gel) shows a binding capacity of about 7.5 mg or more agalacto human Transferrin [agalacto hTF] through its binding to a terminal [GlcNAcβ(1-2)Man] glycan epitope on agalacto hTF.

New Product

Supplier: Cytiva

Description: DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

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Supplier: Cytiva

Description: Sephadex G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

SDS

Supplier: Cytiva

Description: Ni Ni Sepharose High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

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Supplier: Cytiva

Description: Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).

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Supplier: Cytiva

Description: Capto™ S is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

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Supplier: Cytiva

Description: MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

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Catalog Number: (97067-872)

Supplier: Cytiva

Description: Phenyl Sepharose High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.

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Catalog Number: (97067-926)

Supplier: Cytiva

Description: Capto Butyl is a hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification, Their combination of high capacity, high flow rate and low backpressure reduces process cycle times.

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Supplier: Cytiva

Description: Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.

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Catalog Number: (95017-040)

Supplier: Cytiva

Description: Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

SDS Certificates

Catalog Number: (95016-858)

Supplier: Cytiva

Description: Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

SDS Certificates

Catalog Number: (TCR0239-1VIAL)

Supplier: TCI America

Description: The rGRFT is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Griffithsia sp. This lectin preferably binds to a [Manα(1-2)Man] glycan epitope.
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rGRFT-LecBeads (1 ml gel) shows a binding capacity of about 6 mg or more bovine Ribonuclease B [RNase B] through its binding to a terminal [Manα(1-2)Man] glycan epitope on RNase B.

New Product

Catalog Number: (76488-174)

Supplier: Cytiva

Description: Streptavidin Sepharose™ High Performance is an affinity chromatography resin designed for fast and reliable, high resolution purification of biotinylated biomolecules.