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You Searched For: Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-101228-574

Factor Xa Protease, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg

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Image Unavailable-80600-992

PROTEIN GRADE® Detergent, C12E9, Calbiochem®, MilliporeSigma

Catalog Number: (80600-992)
Supplier: MilliporeSigma
Description: A non-ionic detergent used for the solubilization of membrane-bound proteins in their native state.

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Image Unavailable-71003-228

Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences

Catalog Number: (71003-228)
Supplier: G-Biosciences
Description: G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose

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Image Unavailable-75831-572

Lumi Super Tracker™ Soluble/ELISA Chemiluminescent Peroxidase Substrate, ImmunO4

Supplier: Immuno4 LLC
Description: Developed specifically for ELISA and In-Vitro Diagnostic applications requiring ultra sensitivity with exceptional signal to noise ratios.

Image Unavailable-103370-290

VersaLyse™ RBC Lysing Solution, Beckman Coulter

Catalog Number: (103370-290)
Supplier: Beckman Coulter
Description: VersaLyse lysing solution is intended for the lysis of red blood cells in the preparation of biological samples for flow cytometry analysis.
Product Image-PI90305

Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Catalog Number: (PI90305)
Supplier: Invitrogen
Description: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Product Image-PI87779

Pierce™ Detergent Removal Spin Column, Thermo Scientific

Supplier: Invitrogen
Description: Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.

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Product Image-PI90056

Pierce™ Endoproteinase Chymotrypsin Protease (TLCK treated), MS Grade, Thermo Scientific

Catalog Number: (PI90056)
Supplier: Invitrogen
Description: Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.

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Product Image-PI90053

Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific

Catalog Number: (PI90053)
Supplier: Invitrogen
Description: Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications. Store at -20˚C in a nonfrost-free freezer.

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Product Image-PI20343

Pierce™ Immobilized Pepsin (Agarose Resin), Thermo Scientific

Catalog Number: (PI20343)
Supplier: Invitrogen
Description: Immobilized Pepsin agarose consists of an acidic endopeptidase that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.

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Product Image-PI90082

Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Supplier: Invitrogen
Description: Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.

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Product Image-PI90054

Pierce™ Endoproteinase Glu-C Protease, MS Grade, Thermo Scientific

Catalog Number: (PI90054)
Supplier: Invitrogen
Description: Glu-C Protease, MS Grade, is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications, in-solution digestion of proteins and in-gel digestion of proteins. Store at -20 ˚C in a nonfrost-free freezer.

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Product Image-PI88216

Pierce™ Micrococcal Nuclease Solution, Thermo Scientific

Catalog Number: (PI88216)
Supplier: Invitrogen
Description: The Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

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Product Image-PI90067

Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific

Catalog Number: (PI90067)
Supplier: Invitrogen
Description: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling

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Product Image-PI28340

Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific

Catalog Number: (PI28340)
Supplier: Invitrogen
Description: Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.

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Image Unavailable-PI77126

Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific

Supplier: Invitrogen
Description: Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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