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You Searched For: Protein Assays

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-97065-010

VWR® Bradford Method Protein Assay Kit

Supplier: VWR
Description: The Bradford Protein Assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method. The standard protocol is used for measuring 10-100 μg of protein and the microassay is used for measuring 1-10 μg of protein. For maximum convenience, Bradford Reagent and 0.5 mg/ml BSA standard solution are offered individually or as components of a kit.

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Product Image-10147-342

AzureRed Fluorescent Protein Stain, Azure BioSystems

Catalog Number: (10147-342)
Supplier: Azure Biosystems
Description: AzureRed Fluorescent Protein Stain is a quantitative total protein stain for blots and blots with the sensitivity to detect less than 1 ng of protein per band or spot

SDS Product available on GSA Advantage®


Product Image-89165-918

PROTEOSTAT® Thermal Shift Stability Assay Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: This kit has been designed for monitoring protein stability under systematic thermal stress conditions.

Product Image-10033-576

FLUOR DE LYS® SIRT5 fluorometric drug discovery assay kit, Enzo Life Sciences

Catalog Number: (10033-576)
Supplier: Enzo Life Sciences
Description: The SIRT5 Fluorometric Drug Discovery Kit is a sensitive fluorometric assay is designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5.
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Ubiquitin Conjugating Kit (HeLa Lysate-Based), Enzo Life Sciences

Catalog Number: (89165-450)
Supplier: Enzo Life Sciences
Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
Product Image-89156-536

Caspase-1 Assay Kit for Drug Discovery, Enzo Life Sciences

Catalog Number: (89156-536)
Supplier: Enzo Life Sciences
Description: This kit is a complete assay system to measure protease activity of recombinant caspase-1
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HAT Activity Assay Kit, Enzo Life Sciences

Catalog Number: (89156-146)
Supplier: Enzo Life Sciences
Description: Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation
Product Image-89156-498

DPPIV/CD26 Assay Kit for Biological Samples, Enzo Life Sciences

Catalog Number: (89156-498)
Supplier: Enzo Life Sciences
Description: The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered <i>in vivo</i>.
Product Image-89165-248

Auto-Ubiquitinylation Kit, Enzo Life Sciences

Catalog Number: (89165-248)
Supplier: Enzo Life Sciences
Description: This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation
Product Image-89165-452

Ubiquitinylation Kit, Enzo Life Sciences

Catalog Number: (89165-452)
Supplier: Enzo Life Sciences
Description: Versatile tool for generation of ubiquitin-E2 thioesters.
Image Unavailable-76002-366

ADAM17 Fluorometric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (76002-366)
Supplier: Enzo Life Sciences
Description: Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.
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10x Ubiquitinylation Buffer, Enzo Life Sciences

Catalog Number: (89160-402)
Supplier: Enzo Life Sciences
Description: Assay Buffer from the Ubiquitinylation Kits
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PROTEOSTAT® Protein Aggregation Standards, Enzo Life Sciences

Catalog Number: (10033-526)
Supplier: Enzo Life Sciences
Description: This kit is ideal for reliable and accurate quantification of protein aggregation in solution.
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Proteasome 20S Assay Kit, Enzo Life Sciences

Catalog Number: (89156-546)
Supplier: Enzo Life Sciences
Description: A QuantiZyme™ Assay System
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Caspase-2 Fluorometric Assay Kit, Enzo Life Sciences

Catalog Number: (89155-956)
Supplier: Enzo Life Sciences
Description: Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.
Product Image-10033-580

Chemilum de Lys® HDAC/SIRT Chemiluminescent Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (10033-580)
Supplier: Enzo Life Sciences
Description: The Chemilum de Lys® HDAC/SIRT Chemiluminescent Drug Discovery Kit give you all the benefits of chemiluminescent detection without any of the side effects.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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