You Searched For: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.


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Supplier: Promega Corporation
Description: Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1) and MKK6. Overexpression of ASK1 induces apoptotic cell death.

SDS

Catalog Number: (PAV6922)
Supplier: Promega Corporation
Description: Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.

SDS


Catalog Number: (PAV6859)
Supplier: Promega Corporation
Description: Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.

SDS


Catalog Number: (PAV6910)
Supplier: Promega Corporation
Description: Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.

SDS


Catalog Number: (75817-138)
Supplier: Cayman Chemical Company
Description: A kit for measuring ascorbate.


Catalog Number: (75878-142)
Supplier: BIOASSAY SYSTEMS
Description: For quantitative determination of invertase/sucrase activity.


Catalog Number: (75877-992)
Supplier: BIOASSAY SYSTEMS
Description: For quantitative determination of copper(II) ion concentrations.


Catalog Number: (PAV9510)
Supplier: Promega Corporation
Description: The NADPH Regeneration System is an enzyme substrate combination that reduces NADP+ to NADPH.

Catalog Number: (PAV8311)
Supplier: Promega Corporation
Description: Recombinant full-length human ZAP70 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAP70 is a nonreceptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor.


Catalog Number: (75878-094)
Supplier: BIOASSAY SYSTEMS
Description: For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism


Catalog Number: (PAV4079)
Supplier: Promega Corporation
Description: Recombinant full-length human p38 delta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. It is a member of the p38 MAPK family and is activated by chemical and environmental stresses as well as by proinflammatory cytokines.


Catalog Number: (PAV4063)
Supplier: Promega Corporation
Description: Recombinant human FGFR4 (460-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. FGFR4 is a member of the fibroblast growth factor receptor family, which play a role in mitogenesis and differentiation.


Catalog Number: (PAV9221)
Supplier: Promega Corporation
Description: Recombinant full-length human CDK2 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.


Catalog Number: (PAV4167)
Supplier: Promega Corporation
Description: Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.


Catalog Number: (PAV4489)
Supplier: Promega Corporation
Description: Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.


Catalog Number: (PAV4169)
Supplier: Promega Corporation
Description: Recombinant human MET (point mutant M1250T; amino acids 956-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.


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