You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description: Cloning Enzyme Premix, simple rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site, Clone any insert at any site within any vector, Restriction enzyme and phosphatase free system, Size: 10 Rxns
Catalog Number: 104013-320
Supplier: INTACT GENOMICS, INC MS


Description: T4 DNA Ligase, Purity: >/= 99%, Product Source: E. coli strain expressing a recombinant clone, Product Includes: T4 DNA Ligase, 10x T4 DNA Ligase Reaction Buffer, Applications: Cloning of PCR products, Next-gen library, Size: 400, 000 units (2000 units/µl)
Catalog Number: 103753-184
Supplier: INTACT GENOMICS, INC MS


Description: ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 10 reactions
Catalog Number: 103753-208
Supplier: INTACT GENOMICS, INC MS


Description: ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 50 reactions
Catalog Number: 103753-210
Supplier: INTACT GENOMICS, INC MS


Description: ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 100reactions
Catalog Number: 103753-212
Supplier: INTACT GENOMICS, INC MS


Description: T4 DNA Ligase, Purity: >/= 99%, Product Source: E. coli strain expressing a recombinant clone, Product Includes: T4 DNA Ligase, 10x T4 DNA Ligase Reaction Buffer, Applications: Cloning of PCR products, Next-gen library, Size: 100, 000 units (400 units/µl)
Catalog Number: 103753-180
Supplier: INTACT GENOMICS, INC MS


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