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You Searched For: Cloning Buffers

In molecular biology, cloning buffer solutions containing TRIS, acetic acid, EDTA, or more are used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Various buffer formulations are available with different base ingredients in the mixture. These different mixtures have varied buffer capacities and run times. Buffer solutions serve to protect samples against pH changes while the sample is undergoing a wide variety of chemical applications.

In molecular biology, cloning buffer solutions containing TRIS, acetic acid, EDTA, or more are used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Various buffer formulations are available with different base ingredients in the mixture. These different mixtures have varied buffer capacities and run times. Buffer solutions serve to protect samples against pH changes while the sample is undergoing a wide variety of chemical applications.


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Product Image-102855-118

T4 RNA Ligase Reaction Buffer, New England Biolabs

Catalog Number: (102855-118)
Supplier: New England Biolabs (NEB)
Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes

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Image Unavailable-80058-050

EDTA, 500 mM, pH 8.0, Calbiochem®

Catalog Number: (80058-050)
Supplier: MilliporeSigma
Description: Cloning Buffer, EDTA Solution, 500 mm

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Product Image-101227-398

Antarctic Phosphatase Reaction Buffer, New England Biolabs

Catalog Number: (101227-398)
Supplier: New England Biolabs (NEB)
Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes

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Product Image-PAV6232

TE Buffer, 1X, Molecular Biology Grade, Promega

Supplier: Promega Corporation
Description: TE Buffer, 1X, Molecular Grade (pH 8.0), is a buffer composed of 10 mM Tris-HCl containing 1 mM EDTA.Na<sub>2</sub>.

SDS Certificates

Image Unavailable-AAJ62848-AK

Tris(hydroxymethyl)aminomethane hydrochloride, 1M soln., pH 7,4, RNase free, Thermo Scientific

Catalog Number: (AAJ62848-AK)
Supplier: Thermo Scientific Chemicals
Description: Liquid

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Image Unavailable-AAJ63240-AP

Thrombin Buffer, pH 8.0, Thermo Scientific

Catalog Number: (AAJ63240-AP)
Supplier: Thermo Scientific Chemicals
Description: Liquid

SDS Certificates


Product Image-103305-700

EDTA Buffer (10X), pH 8.5, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences
Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

Image Unavailable-AAJ62907-AK

Alkaline Phosphatase Buffer-1 (5X), Thermo Scientific

Supplier: Thermo Scientific Chemicals
Description: Liquid

SDS Certificates

Product Image-PAA2631

EDTA 10 mM, (pH 8.0), Promega

Catalog Number: (PAA2631)
Supplier: Promega Corporation
Description: EDTA is a chelator of divalent cations suitable for biochemistry and molecular biology applications.

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Product Image-PAC1313

T4 Polynucleotide Kinase Buffer Pack

Catalog Number: (PAC1313)
Supplier: Promega Corporation
Description: Designed for use with T4 Polynucleotide Kinase to catalyze the transfer of the γ-phosphate from ATP to the 5´-terminus of poly- or mononucleotides bearing a 5´-hydroxyl group.

Certificates


Image Unavailable-AAJ60832-AK

Potassium acetate, 1M aqua solution, pH 7.5, RNAse free, Thermo Scientific

Catalog Number: (AAJ60832-AK)
Supplier: Thermo Scientific Chemicals
Description: Cloning Buffer

SDS Certificates


Image Unavailable-AAJ61928-AK

Sodium Acetate, 3M aqua solution, pH 5.2, RNAse free, Thermo Scientific

Supplier: Thermo Scientific Chemicals
Description: Sodium acetate buffer solution pH, 5.2 is been widely used in for precipitation of nucleic acids and preparation of gel stains for protein gel electrophoresis. Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as Chip-on-Chip assay and in vitro enzyme inhibition assay.

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Image Unavailable-AAJ62052-AE

Sodium pyrophosphate, 200 mM buffer solution., Thermo Scientific

Supplier: Thermo Scientific Chemicals
Description: Liquid

SDS Certificates

Image Unavailable-AAJ60080-AK

Buffer, Tris(hydroxymethyl)aminomethane Hydrochloride, 1M Solution, pH 8,0, RNase-free, Thermo Scientific

Catalog Number: (AAJ60080-AK)
Supplier: Thermo Scientific Chemicals
Description: Liquid

SDS Certificates


Product Image-101227-374

Taq DNA Ligase Reaction Buffer, New England Biolabs

Catalog Number: (101227-374)
Supplier: New England Biolabs (NEB)
Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes

Small Business Enterprise


Product Image-101227-368

T4 DNA Ligase Reaction Buffer, New England Biolabs

Catalog Number: (101227-368)
Supplier: New England Biolabs (NEB)
Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes

Small Business Enterprise


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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