You Searched For: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE)

Catalog Number: (89139-558)

Supplier: Biotium

Description: 5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate.

Supplier: AAT BIOQUEST INC

Description: Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations.

Supplier: Ambeed

Description: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester, Purity: 98% (isomer mixture), CAS Number: 117548-22-8, Appearance: Off-white to orange Powder or crystals, Storage: Inert atmosphere, Store in freezer, under -20C, Size: 1MG

Supplier: Invitrogen

Description: NHS fluorescein [(5/6-carboxyfluorescein succinimidyl ester), mixed isomer] is an amine reactive derivative of fluorescein dye for antibodies and other probes in fluorescence microscopy, flow cytometry, and immunofluorescence based assays such as western blotting and ELISA.

Certificates

Supplier: Sigma Aldrich

Description: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.

Supplier: Chem Impex International

Supplier: Anaspec Inc

Description: Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.