Pierce™ Immunoprecipitation, Direct IP Kit, Thermo Scientific

Supplier: LIFE TECHNOLOGIES MS

26148
PI26148EA 589.77 USD
PI26148
Pierce™ Immunoprecipitation, Direct IP Kit, Thermo Scientific
Protein Purification Protein Purification Kits

The Pierce™ Direct IP Kit uses an activated resin to covalently immobilize IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.


  • Requires less than 10 µg of antibody for a single IP experiment
  • Eliminates antibody contamination
  • Immobilize 100 to 200 µg of antibody to prepare ready-made IP affinity resin
  • Spin columns eliminate resin loss and provide for more efficient separation of solutions
  • Use chicken IgY, human IgE, mouse IgM or any other purified protein


The primary benefits resulting from this method are the opportunity to use any species or subclass of purified antibody (not just types that bind to Protein A or G) and the ability to purify target protein without contamination by the antibody. The method also makes it possible to immunoprecipitate antigens from serum samples without co-purifying non-target immunoglobulins. Finally, the kit uses microcentrifuge spin cups to effectively wash and separate samples from the beaded agarose resin.


Información de suministro: Kit includes AminoLink™ Plus coupling resin (2 mL), coupling buffer 20X, (25 mL), quenching buffer (50 mL), wash solution (50 mL), sodium cyanoborohydride solution 5M, (0.5 mL), IP lysis/wash buffer (2x50 mL), conditioning buffer 100X, (5 mL), tris-buffered saline 20X, (25 mL), elution buffer (50 mL), lane marker sample buffer 5X, (5 mL), control agarose resin (2 mL), spin columns and collection tubes.


Precaución: For Research Use Only. Not for use in diagnostic procedures.

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