DTSSP (3,3'-Dithiobis(sulfosuccinimidyl propionate)), Pierce™

Supplier: LIFE TECHNOLOGIES MS

Synonyms: DTSSP

21578
PI21578EA 203.08 USD
PI21578
DTSSP (3,3'-Dithiobis(sulfosuccinimidyl propionate)), Pierce™
DTSSP (3,3'-Dithiobis(sulfosuccinimidyl propionate))

Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.


  • Reactive groups: sulfo-NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
  • Spacer arm also easily cleaved with reducing SDS-PAGE sample loading
  • Cleavable crosslinker allows separation of crosslinked products
  • Water-soluble; compare with DSP
  • Membrane-impermeable, allowing for cell surface labeling


DTSSP is 3,3'-Dithiobis(sulfosuccinimidylpropionate), which contains an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker.


Precaución: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₁₄H₁₆N₂O₁₄S₄
MW: 564.542 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD01861949
CAS Number: 81069-02-5

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Specification Test Results

Product Line Pierce™
Labeling Method Chemical Labeling
Crosslinker Type Homobifunctional
Reactive Moiety Sulfo-NHS Ester
Spacer Cleavable, Long
Form Powder
Quantity 50 mg
Type Crosslinker
Solubility Water

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